A Beginner’s Guide to PCR Testing

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With a definitive cure or vaccine still unavailable to the public, healthcare professionals have advised that at the moment, mass testing is the most ideal strategy in controlling the coronavirus outbreak. Last April 24, the The Inter-Agency Task Force – National Task Force (IATF-NTF) and the DOH have announced that the RT-PCR testing for COVID-19 will be significantly expanded. From the current average of 4,500 PCR tests conducted per day, the government wishes to be able to do at least 30,000 tests per day by May 30, 2020. As the most trusted method both by the DOH and most medical professionals, what exactly is PCR testing and why is it deemed by medical experts as the most reliable testing method?

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PCR stands for polymerase chain reaction and has been a recognized and widely used molecular method since the 1980’s. It allows those at the lab to extract from swab samples and extract a viral genetic material called RNA. This material is necessary to create a complimentary strand of DNA which is crucial for the next stage. Extraction usually takes less than 30 minutes.

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NOW AVAILABLE: GeneFinder EX-MATE 32 Plus Nucleic Acid Extraction System

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The COVID-19 virus has 30,000 nucleotides, the sort of building blocks that composes the RNA and DNA. According to a study done by researchers in the Clinical Virology Laboratory at UW Medicine, there are 100 nucleotides that are unique in the COVID-19 virus. Within those 100 nucleotides, there are specific genes that the PCR method will look for to definitively determine the presence of the virus.

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The constructed DNA strand from the extraction process will be amplified through a technique called reverse transcriptase polymerase chain reaction (RT-PCR). Through amplification, the PCR machine makes thousands of copies of the DNA strand to make it measurable and easier to study. PCR machines differ in which specific COVID-19 genes they would target (RdRp gene, N gene, E gene). If two or more of these genes are confirmed to be within the sample, the person under investigation is verified to be a carrier of the coronavirus disease.

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NOW AVAILABLE: GeneFinder COVID-19 Plus RealAmp Kit

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The DOH relies on PCR method for confirmatory COVID-19 testing.What makes the PCR testing the method of choice for most medical professionals is that it is both specific and sensitive, with most PCR machines’s sensitivity and specificity being 90% and above . This is why, in accordance to the guidelines released by DOH, rapid antibody tests must still be conducted in conjunction with PCR tests. The level of accuracy it offers is greatly needed, especially at a time where diagnostic certainty is at its most crucial.

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MAN Pharma has recently released a PCR testing kit for COVID-19. Unlike most PCR machines, it targets all three genes which ensures higher diagnostic accuracy. It is compatible with ABI 7500, ABI7500 FAST, and CFX96 PCR models. If you are interested in availing this kit, kindly fill out the quotation request form below.

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Sources:
Government Launches Public-Private Task Fforce T3 (Test, Trace and Treat) to Significantly Expand RT-PCR Testing Capacity – The Department of Health
Interim Guidelines on Expanded Testing for COVID-19 – The Department of Health
Here’s How Coronavirus Tests Work—and Who Offers Them – Scientific American
How do the new coronavirus tests work? – Live Science
COVID-19 Diagnostics Explained – Asian Scientist Magazine
UW Medicine gets green light to test for coronavirus – Seattle Times

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MAN Pharma Launches COVID-19 PCR Test Kits

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MAN Pharma wishes to announce that we will be introducing the GeneFinder™ COVID-19 Plus RealAmp Kit to the Filipino healthcare market. Created by the renowned South Korean manufacturer OSANG Healthcare, this FDA-approved PCR kit is widely used in affected countries such as Italy, Romania, Morocco, Brazil, USA, and Russia.
This one-step reverse transcription real-time PCR kit is designed to qualitatively detect the novel coronavirus (COVID-19) through reverse transcription reaction and real-time polymerase chain reaction. Through its 4-step process, the test kit can detect the virus in just 120 minutes with undisputed accuracy. This is achieved through the amplification of RdRp gene, E gene, and N gene in accordace to the WHO recommended protocol. It can test for respiratory specimen, which includes sputum, nasopharyngeal swab, and oropharyngeal swab. The test kit contains 100 tests and is compatible with ABI 7500, ABI7500 FAST, and CFX96 PCR models.
For more information, kindly refer to our product brochure.

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For hospitals and healthcare providers interested in purchasing this product, we’ve attached a quotation request form below.

 

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What the Coronavirus Means for People with Diabetes

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As the coronavirus outbreak continues to spread across the Philippines, health experts warn us that people with pre-existing conditions must exercise increased precautions against the disease. While they are not more likely to catch the virus, the complications that they may experience when they do get it are far more serious and possibly fatal than COVID-19 patients without pre-existing conditions.

For persons with diabetes, the disease can cause their blood sugar to fluctuate. As a way to give the body the energy it needs to fight off the virus, stored glucose is automatically released into the bloodstream. For persons with diabetes, however, the incapability to produce the necessary insulin to cope with this could trigger the blood sugars to rise. The continuous and often uncontrollable shift in blood sugar levels during this time could cause long-term harm to a person with diabetes’ health, particularly for their eyes, feet and other areas of the body.

That is why, aside from the generally advised social distancing and hygienic guidelines, people with diabetes must practice these added protective measures to keep themselves healthy and safe from the COVID-19.

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Be in constant contact with your diabetes adviser or nurse.

Follow their advice, particularly in regards to your medication. In keeping with social distancing protocols, it is best to do consultations via calls and emails. Do not go to the pharmacy or hospital unless it is absolutely necessary.

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Observe your diet and stay hydrated.

Drink lots of unsweetened drinks. Eat less and often.

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Watch out for signs of hyperglycemia.

If you do not monitor your glucose regularly, observe your own health status. If you are urinating more than usual (especially at night), constantly thirsty, and experiencing headaches, tiredness, and lethargy, consult your health adviser immediately.

 

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Check your blood sugar more frequently.

If you do perform regular glucose monitoring, consult your adviser if you’d need to have it checked more often than usual. This is especially important if you are a PUI or PUM. It is advisable for people with Type 1 diabetes to have theirs checked 4 times daily, including at night. If your glucose level is noticeably high, notify your health adviser immediately.

 

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For people with diabetes, keeping the sugar level in check is more important than ever. Manage your diabetes monitoring with ease and comfort with the GluNEO Lite glucose meter. If you’re interested in purchasing our glucose meter, simply accomplish the form below to request for a quotation.

 

Sources:
Updates: Coronavirus and diabetes – Diabetes UK
Diabetes and COVID-19 – Endocrine Web

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